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Cracking the regulatory genome: Which genetic variants in DNase I sensitive regions are functional?

Overview

We developed a novel approach to annotating functional regulatory regions that integrates sequence information (e.g., position weight matrices) with DNaseI footprinting data to predict the impact of a sequence change on TF binding. We identified over 3 million regulatory variants predicted to affect active regulatory regions for a panel of TFs in over 650 cell-types.

Moyerbrailean GA, Kalita CA, Harvey CT, Wen X, Luca F, Pique-Regi R. (2016) Which Genetics Variants in DNase-Seq Footprints Are More Likely to Alter Binding? PLoS Genet 12(2): e1005875. doi: 10.1371/journal.pgen.1005875

USCS Browser Hub

We have provided annotations as a brower hub to facilitate viewing of the data. There are two tracks: 1) regulatory variants, and 2) regulatory footprints.

Launch UCSC browser with hub (new window)

Data

Regulatory Regions

The footprint annotation data is also available as bed files, separated both by cell-type and TF motif. Each file is formatted as bed9+ files, with four additional data fields: SNP position, 1KG reference allele, 1KG alternate allele, prior log ratio for binding of the reference allele, and the prior LR for binding of the alternate allele. For TFBS without a polymorphism, the SNP position is listed as "0" and the prior LR for the reference allele is repeated.

Supplemental Files

The supplemental excel files associated with the above publications can be downloaded here:

Additional Files

Acknowledgements

Contact Information

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